SQ1002
| SQ1002_RNA | |
|---|---|
 Consensus secondary structure and sequence conservation of Clostridioides difficile sRNA SQ1002 | |
| Identifiers | |
| Symbol | SQ1002_RNA |
| Rfam | RF03533 |
| Other data | |
| RNA type | Gene; sRNA |
| GO | GO:0070928 |
| SO | SO:0000370 |
| PDB structures | PDBe |
SQ1002 is a 191nt small RNA identified in Clostridioides difficile 630 by RNA deep sequencing and confirmed by Northern blot and RT-PCR.[1] Extremities were confirmed by 5’ and 3’-RACE.
This RNA is found in several C. difficile strains at syntenic positions between a putative acyltransferase gene and a ferrous iron transport gene. No homologs were found outside C. difficile, although a putative paralog was found in C. difficile 630, located between a tryptophanyl-tRNA synthetase gene and a pseudogene.
Due to lack of sequence variation, the proposed secondary structure is not supported by any covariation and should be considered speculative.
References[edit]
- ^ Soutourina OA, Monot M, Boudry P, Saujet L, Pichon C, Sismeiro O; et al. (2013). "Genome-wide identification of regulatory RNAs in the human pathogen Clostridium difficile". PLOS Genet. 9 (5): e1003493. doi:10.1371/journal.pgen.1003493. PMC 3649979. PMID 23675309.
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