Partial specific volume
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The partial specific volume express the variation of the extensive volume of a mixture in respect to composition of the masses. It is the partial derivative of volume with respect to the mass of the component of interest.
- Failed to parse (SVG (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "http://localhost:6011/en.wikipedia.org/v1/":): {\displaystyle V=\sum _{i=1}^n m_i \bar{v_i},}
where is the partial specific volume of a component defined as:
The PSV is usually measured in milliLiters (mL) per gram (g), proteins > 30 kDa can be assumed to have a partial specific volume of 0.708 mL/g.[1] Experimental determination is possible by measuring the natural frequency of a U-shaped tube filled successively with air, buffer and protein solution.[2]
Properties[edit]
The weighted sum of partial specific volumes of a mixture or solution is an inverse of density of the mixture namely the specific volume of the mixture.
See also[edit]
References[edit]
- ^ Buxbaum, Engelbert (2011). "25: Centrifugation". Biophysical Chemistry of Proteins. New York, Dordrecht, Heidelberg, London: Springer. pp. 237–249. doi:10.1007/978-1-4419-7251-4_25. ISBN 978-1-4419-7250-7.
- ^ Kratky, O.; Leopold, H.; Stabinger, H. (1973). "5: The determination of the partial specific volume of proteins by the mechanical oscillator technique". In Hirs, C.H.W.; Timasheff, S.N. (eds.). Meth. Enzymol. Vol. 27: Enzyme Structure Part D. pp. 98–110. ISBN 978-0-12-181890-6.