Metabacillus

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Metabacillus
Scientific classification Edit this classification
Domain: Bacteria
Phylum: Bacillota
Class: Bacilli
Order: Bacillales
Family: Bacillaceae
Genus: Metabacillus
Patel and Gupta 2020
Species

Information based on LPSN 2021

Metabacillus is a genus of rod-shaped bacteria exhibiting Gram-positive or Gram-variable staining in the family Bacillaceae within the order Bacillales.[1][2] The type species for this genus is Metabacillus fastidiosus.[1]

This genus was demarcated from the phylogenetically complicated genus Bacillus, whose unclear evolutionary relationships have been long been under scrutiny by the scientific community.[2][3][4] The polyphyletic nature of the genus Bacillus is partly due to the vague criteria used to assign novel bacteria to this genus, resulting in an agglomeration of phylogenetically unrelated bacteria with a diverse range of biochemical characteristics.[2] Comparative genomic studies and phylogenetic analyses have been published to clarify the complicated relationships within this genus resulting in the transfer of many Bacillus species into novel genera such as Alkalihalobacillus,[1] Virigibacillus,[5] Brevibacillus,[6] Solibacillus[7] and Peribacillus.[8] In addition, the genus itself has been restricted to only include species closely related to its type species, Bacillus subtilis as well as Bacillus cereus.[1][8]

The name Metabacillus is derived from its taxonomic position identified through phylogenetic trees. The prefix "meta-" comes from the Greek adjective meta, and translates to "beside". Bacillus comes from the Latin noun bacillus, referring to both 'a small staff or rod' and Bacillus, the bacterial genus. Put together, the name Metabacillus refers to a genus besides Bacillus.[1]

Biochemical characteristics and molecular signatures[1][edit]

All members of the genus Metabacillus are aerobic and demonstrates endospore formation. These bacteria can be found in a wide range of environments, from hypersaline lakes, coastal marine regions and soil. While some species are non-motile, most Metabacillus species are motile. Some species are moderately salt tolerant and able to live in saline environments such as coastal regions. Metabacillus can survive in temperatures ranging 4–45 °C (39–113 °F), but optimal growth occurs in the range of 25–37 °C (77–99 °F).

Six conserved signature indels (CSIs) have been identified as specific for Metabacillus species in proteins such as 3-phosphoshikimate-1-carboxyvinyltransferase, fibronectin/fibrinogen-binding protein, spore protease YyaC, and DEAD/DEAH box helicase.[1] These CSIs were identified through the analysis of genome sequences from Metabacillus species and provide a reliable means to distinguish Metabacillus from other Bacillaceae genera in molecular terms.

Taxonomy[edit]

As of May 2021, there are a total of 15 species with validly published names in the genus Metabacillus.[9] Members of this genus group together and forms a monophyletic branch in phylogenetic trees created from concatenated sequences from various datasets of conserved proteins.[1] This monophyletic clade is also found in the Genome Taxonomy Database (GTDB).[10]

Additional phylogenetic studies have identified the species Bacillus weihaiensis as a member of Metabacillus based on taxonomic placement in phylogenetic trees as well as shared molecular markers (specifically conserved signature indels) with other members of Metabacillus.[8] However, transfer was not proposed due to the lack of strain culture information. As Bacillus continues to be a fast growing genus with the frequent addition of novel species and sequenced genomes, it would be necessary to continually update and validate previous classification proposals.

References[edit]

  1. ^ a b c d e f g h Patel, Sudip; Gupta, Radhey S. (2020-01-01). "A phylogenomic and comparative genomic framework for resolving the polyphyly of the genus Bacillus: Proposal for six new genera of Bacillus species, Peribacillus gen. nov., Cytobacillus gen. nov., Mesobacillus gen. nov., Neobacillus gen. nov., Metabacillus gen. nov. and Alkalihalobacillus gen. nov". International Journal of Systematic and Evolutionary Microbiology. 70 (1): 406–438. doi:10.1099/ijsem.0.003775. ISSN 1466-5026. PMID 31617837.
  2. ^ a b c Brenner, Don J.; Krieg, Noel R.; Staley, James T.; Garrity, George M.; Boone, David R.; De Vos, Paul; Goodfellow, Michael; Rainey, Fred A.; Schleifer, Karl-Heinz, eds. (2005). Bergey's Manual® of Systematic Bacteriology. doi:10.1007/0-387-28022-7. ISBN 978-0-387-24144-9.
  3. ^ Logan, N.A. (2011-12-20). "Bacillus and relatives in foodborne illness". Journal of Applied Microbiology. 112 (3): 417–429. doi:10.1111/j.1365-2672.2011.05204.x. ISSN 1364-5072. PMID 22121830.
  4. ^ La Duc, Myron T; Satomi, Masataka; Agata, Norio; Venkateswaran, Kasthuri (March 2004). "gyrB as a phylogenetic discriminator for members of the Bacillus anthracis–cereus–thuringiensis group". Journal of Microbiological Methods. 56 (3): 383–394. doi:10.1016/j.mimet.2003.11.004. ISSN 0167-7012. PMID 14967230.
  5. ^ Heyndrickx, M.; Lebbe, L.; Kersters, K.; Hoste, B.; De Wachter, R.; De Vos, P.; Forsyth, G.; Logan, N. A. (1999-07-01). "Proposal of Virgibacillus proomii sp. nov. and emended description of Virgibacillus pantothenticus (Proom and Knight 1950) Heyndrickx et al. 1998". International Journal of Systematic and Evolutionary Microbiology. 49 (3): 1083–1090. doi:10.1099/00207713-49-3-1083. ISSN 1466-5026. PMID 10425765.
  6. ^ Shida, O.; Takagi, H.; Kadowaki, K.; Komagata, K. (1996-10-01). "Proposal for Two New Genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov". International Journal of Systematic Bacteriology. 46 (4): 939–946. doi:10.1099/00207713-46-4-939. ISSN 0020-7713. PMID 8863420.
  7. ^ Mual, Poonam; Singh, Nitin Kumar; Verma, Ashish; Schumann, Peter; Krishnamurthi, Srinivasan; Dastager, Syed; Mayilraj, Shanmugam (2016-05-01). "Reclassification of Bacillus isronensis Shivaji et al. 2009 as Solibacillus isronensis comb. nov. and emended description of genus Solibacillus Krishnamurthi et al. 2009". International Journal of Systematic and Evolutionary Microbiology. 66 (5): 2113–2120. doi:10.1099/ijsem.0.000982. ISSN 1466-5026. PMID 26907585.
  8. ^ a b c Gupta, Radhey S.; Patel, Sudip; Saini, Navneet; Chen, Shu (2020-11-01). "Robust demarcation of 17 distinct Bacillus species clades, proposed as novel Bacillaceae genera, by phylogenomics and comparative genomic analyses: description of Robertmurraya kyonggiensis sp. nov. and proposal for an emended genus Bacillus limiting it only to the members of the Subtilis and Cereus clades of species". International Journal of Systematic and Evolutionary Microbiology. 70 (11): 5753–5798. doi:10.1099/ijsem.0.004475. ISSN 1466-5026. PMID 33112222.
  9. ^ "Genus: Metabacillus". lpsn.dsmz.de. Retrieved 2021-05-20.
  10. ^ "GTDB - Tree". gtdb.ecogenomic.org. Retrieved 2021-05-25.
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