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Protein-coding gene in the species Homo sapiens
Dr1-associated corepressor is a protein that in humans is encoded by the DRAP1 gene .[5] [6]
Transcriptional repression is a general mechanism for regulating transcriptional initiation in organisms ranging from yeast to humans. Accurate initiation of transcription from eukaryotic protein-encoding genes requires the assembly of a large multiprotein complex consisting of RNA polymerase II and general transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a repressor that interacts with the TATA-binding protein (TBP) of TFIID and prevents the formation of an active transcription complex by precluding the entry of TFIIA and/or TFIIB into the preinitiation complex. The protein encoded by this gene is a corepressor of transcription that interacts with DR1 to enhance DR1-mediated repression. The interaction between this corepressor and DR1 is required for corepressor function and appears to stabilize the TBP-DR1-DNA complex.[6]
Interactions [ edit ] DRAP1 has been shown to interact with FOXH1 [7] DR1 .[8] [9]
References [ edit ]
^ a b c GRCh38: Ensembl release 89: ENSG00000175550 – Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000024914 – Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Mermelstein F, Yeung K, Cao J, Inostroza JA, Erdjument-Bromage H, Eagelson K, et al. (April 1996). "Requirement of a corepressor for Dr1-mediated repression of transcription" . Genes & Development . 10 (8): 1033–48. doi :10.1101/gad.10.8.1033 PMID 8608938 . ^ a b "Entrez Gene: DRAP1 DR1-associated protein 1 (negative cofactor 2 alpha)" .^ Iratni R, Yan YT, Chen C, Ding J, Zhang Y, Price SM, et al. (December 2002). "Inhibition of excess nodal signaling during mouse gastrulation by the transcriptional corepressor DRAP1". Science . 298 (5600): 1996–9. Bibcode :2002Sci...298.1996I . doi :10.1126/science.1073405 . PMID 12471260 . S2CID 22923124 . ^ Mermelstein F, Yeung K, Cao J, Inostroza JA, Erdjument-Bromage H, Eagelson K, et al. (April 1996). "Requirement of a corepressor for Dr1-mediated repression of transcription" . Genes & Development . 10 (8): 1033–48. doi :10.1101/gad.10.8.1033 PMID 8608938 . ^ Yeung K, Kim S, Reinberg D (January 1997). "Functional dissection of a human Dr1-DRAP1 repressor complex" . Molecular and Cellular Biology . 17 (1): 36–45. doi :10.1128/MCB.17.1.36 . PMC 231727 PMID 8972183 .
Further reading [ edit ]
Goppelt A, Stelzer G, Lottspeich F, Meisterernst M (June 1996). "A mechanism for repression of class II gene transcription through specific binding of NC2 to TBP-promoter complexes via heterodimeric histone fold domains" . The EMBO Journal . 15 (12): 3105–16. doi :10.1002/j.1460-2075.1996.tb00673.x . PMC 450253 PMID 8670811 . Yeung K, Kim S, Reinberg D (January 1997). "Functional dissection of a human Dr1-DRAP1 repressor complex" . Molecular and Cellular Biology . 17 (1): 36–45. doi :10.1128/MCB.17.1.36 . PMC 231727 PMID 8972183 . Ikeda K, Halle JP, Stelzer G, Meisterernst M, Kawakami K (January 1998). "Involvement of negative cofactor NC2 in active repression by zinc finger-homeodomain transcription factor AREB6" . Molecular and Cellular Biology . 18 (1): 10–8. doi :10.1128/mcb.18.1.10 . PMC 121442 PMID 9418848 . Castaño E, Gross P, Wang Z, Roeder RG, Oelgeschläger T (June 2000). "The C-terminal domain-phosphorylated IIO form of RNA polymerase II is associated with the transcription repressor NC2 (Dr1/DRAP1) and is required for transcription activation in human nuclear extracts" . Proceedings of the National Academy of Sciences of the United States of America . 97 (13): 7184–9. Bibcode :2000PNAS...97.7184C . doi :10.1073/pnas.140202297 PMC 16520 PMID 10852970 . Kamada K, Shu F, Chen H, Malik S, Stelzer G, Roeder RG, et al. (July 2001). "Crystal structure of negative cofactor 2 recognizing the TBP-DNA transcription complex" . Cell . 106 (1): 71–81. doi :10.1016/S0092-8674(01)00417-2 PMID 11461703 . S2CID 11064090 . Iratni R, Yan YT, Chen C, Ding J, Zhang Y, Price SM, et al. (December 2002). "Inhibition of excess nodal signaling during mouse gastrulation by the transcriptional corepressor DRAP1". Science . 298 (5600): 1996–9. Bibcode :2002Sci...298.1996I . doi :10.1126/science.1073405 . PMID 12471260 . S2CID 22923124 . Denko N, Wernke-Dollries K, Johnson AB, Hammond E, Chiang CM, Barton MC (February 2003). "Hypoxia actively represses transcription by inducing negative cofactor 2 (Dr1/DrAP1) and blocking preinitiation complex assembly" . The Journal of Biological Chemistry . 278 (8): 5744–9. doi :10.1074/jbc.M212534200 PMID 12477712 . Leonard D, Ajuh P, Lamond AI, Legerski RJ (September 2003). "hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing". Biochemical and Biophysical Research Communications . 308 (4): 793–801. CiteSeerX 10.1.1.539.8359 doi :10.1016/S0006-291X(03)01486-4 . PMID 12927788 . Klejman MP, Pereira LA, van Zeeburg HJ, Gilfillan S, Meisterernst M, Timmers HT (November 2004). "NC2alpha interacts with BTAF1 and stimulates its ATP-dependent association with TATA-binding protein" . Molecular and Cellular Biology . 24 (22): 10072–82. doi :10.1128/MCB.24.22.10072-10082.2004 . PMC 525489 PMID 15509807 . Lewis BA, Sims RJ, Lane WS, Reinberg D (May 2005). "Functional characterization of core promoter elements: DPE-specific transcription requires the protein kinase CK2 and the PC4 coactivator" . Molecular Cell . 18 (4): 471–81. doi :10.1016/j.molcel.2005.04.005 PMID 15893730 . Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, et al. (September 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell . 122 (6): 957–68. doi :10.1016/j.cell.2005.08.029 . hdl :11858/00-001M-0000-0010-8592-0 PMID 16169070 . S2CID 8235923 . Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, et al. (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature . 437 (7062): 1173–8. Bibcode :2005Natur.437.1173R . doi :10.1038/nature04209 . PMID 16189514 . S2CID 4427026 . Assmann EM, Alborghetti MR, Camargo ME, Kobarg J (April 2006). "FEZ1 dimerization and interaction with transcription regulatory proteins involves its coiled-coil region" . The Journal of Biological Chemistry . 281 (15): 9869–81. doi :10.1074/jbc.M513280200 PMID 16484223 . Lim J, Hao T, Shaw C, Patel AJ, Szabó G, Rual JF, et al. (May 2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration" . Cell . 125 (4): 801–14. doi :10.1016/j.cell.2006.03.032 PMID 16713569 . S2CID 13709685 . Albert TK, Grote K, Boeing S, Stelzer G, Schepers A, Meisterernst M (June 2007). "Global distribution of negative cofactor 2 subunit-alpha on human promoters" . Proceedings of the National Academy of Sciences of the United States of America . 104 (24): 10000–5. Bibcode :2007PNAS..10410000A . doi :10.1073/pnas.0703490104 PMC 1891239 PMID 17548813 .
